polyclonal rabbit anti rpap3 antibodies  (Bethyl)

Journal: Marine Drugs

Article Title: Target Identification of the Marine Natural Products Dictyoceratin-A and -C as Selective Growth Inhibitors in Cancer Cells Adapted to Hypoxic Environments

doi: 10.3390/md17030163

Figure Lengend Snippet: Binding of probe A ( 3 ) to the candidate proteins in cell lysates. Cell lysates (100 μg protein each), prepared from DU145 cells cultured under hypoxic conditions (H, lanes 2, 4, and 6) or normoxic conditions (N, lanes 1, 3, and 5) were incubated with probe A ( 3 ). The probe with bound protein was then separated from the supernatant by the addition of streptavidin-conjugated Dynabeads. The resulting supernatants and Dynabeads were used for western blotting analysis to detect RBM28, RPAP3, MIA3, EIF5AL1, and TRMT6.

Article Snippet: Polyclonal rabbit anti-RPAP3 antibodies were obtained from Bethyl Laboratories, Inc. (Montgomery, TX, USA).

Techniques: Binding Assay, Cell Culture, Incubation, Western Blot

Journal: Marine Drugs

Article Title: Target Identification of the Marine Natural Products Dictyoceratin-A and -C as Selective Growth Inhibitors in Cancer Cells Adapted to Hypoxic Environments

doi: 10.3390/md17030163

Figure Lengend Snippet: Growth of RBM28-, RPAP3-, and MIA30-knockdown DU145 cells under normoxic and hypoxic conditions. ( a ) The expression levels of the indicated proteins in each knockdown cell line were analyzed by western blotting. The expression level of each protein was calculated from its relative density, which was normalized to that of β-actin. ( b ) The proliferation rates of MIA3-, RBM28-, and RPAP3-knockdown DU145 cells were investigated under normoxic and hypoxic conditions. The growth inhibition rate was calculated as a percentage relative to the negative control cells. Differences were considered significant at * p < 0.01.

Article Snippet: Polyclonal rabbit anti-RPAP3 antibodies were obtained from Bethyl Laboratories, Inc. (Montgomery, TX, USA).

Techniques: Knockdown, Expressing, Western Blot, Inhibition, Negative Control

Journal: Marine Drugs

Article Title: Target Identification of the Marine Natural Products Dictyoceratin-A and -C as Selective Growth Inhibitors in Cancer Cells Adapted to Hypoxic Environments

doi: 10.3390/md17030163

Figure Lengend Snippet: Expression of hypoxia-related proteins in RPAP3-knockdown DU145 cells. The expression levels of the indicated hypoxia-related proteins in RPAP3-knockdown DU145 cells were compared with those of wild-type DU145 cells treated with/without dictyoceratin-A ( 1 ) or -C ( 2 ) (10 µM each) using western blotting analysis. The expression level of each protein was calculated from its relative density, which was normalized to that of β-actin.

Article Snippet: Polyclonal rabbit anti-RPAP3 antibodies were obtained from Bethyl Laboratories, Inc. (Montgomery, TX, USA).

Techniques: Expressing, Knockdown, Western Blot

Journal: Marine Drugs

Article Title: Target Identification of the Marine Natural Products Dictyoceratin-A and -C as Selective Growth Inhibitors in Cancer Cells Adapted to Hypoxic Environments

doi: 10.3390/md17030163

Figure Lengend Snippet: Effect of dictyoceratin-A ( 1 ) on RPAP3-overexpressing DU145 cells. ( a ) The expression levels of RPAP3 protein in stable RPAP3-overexpressing cells were compared with those in wild-type DU145 cells using western blotting. The expression levels of RPAP3 were calculated based on the relative density of bands, normalized to that of β-actin. ( b ) Antiproliferative activity of dictyoceratin-A ( 1 ) in wild-type and RPAP3-overexpressing DU145 cells. The growth inhibition rate was calculated as a percentage relative to that in control cells. Differences were considered significant at * p < 0.01.

Article Snippet: Polyclonal rabbit anti-RPAP3 antibodies were obtained from Bethyl Laboratories, Inc. (Montgomery, TX, USA).

Techniques: Expressing, Western Blot, Activity Assay, Inhibition, Control